Vitamin B₁₂ Attenuates Acute Pancreatitis by Suppressing Oxidative Stress and Improving Mitochondria Dysfunction via CBS/SIRT1 Pathway

Figure 6

VB 12 also activates the SIRT1 pathway in vivo of acute pancreatitis mice. Animals were randomly divided into four experimental groups: AP group treated with NaT (3%), NaT + VB 12 (20 μg/kg, ip), NaT + VB 12 (40 μg/kg, ip), and control group treated with 0.9% saline (). NaT (3%) was injected through the pancreaticobiliary tract, and pancreatic tissue and blood were collected at 24 hours for follow-up studies. (a, b) The expression of SIRT1, PGC1-α, Nrf2, p-P38, P38, and mitophagy-related proteins in pancreatic tissue was measured by Western blot. Con: control group; NaT: acute pancreatitis group. All the experiments were repeated three times with similar results.

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