Testis-Specific Thioredoxins TXNDC2, TXNDC3, and TXNDC6 Are Expressed in Both Testicular and Systemic DLBCL and Correlate with Clinical Disease Presentation

Table 2

Antibodies used in immunostaining methods and Western blotting analysis. The dilute for all antibodies used in Western blotting analysis was 5% BSA. Trx: thioredoxin; TXNDC: thioredoxin domain-containing protein; 8-OHdG: 8-hydroxy-2-deoxyguanosine; RT: room temperature; m/w: microwaved.

		Immunohistochemistry			Western blotting
Antibody	Source of antibody	Concentration	Incubation	m/w	Dilution
Trx	#2429, Thioredoxin 1 (C63C6) Cell Signaling Technology Inc., Danvers, MA, USA	1 : 400	1 h RT	Tris-EDTA pH 9, 10 min	—
TXNDC2	NBP1-80724; Novus Biologicals, Littleton, CO, USA	1 : 100	1 h RT	Tris-EDTA pH 9, 10 min	1 : 500
TXNDC3	NBP1-85616; Novus Biologicals, Littleton, CO, USA	1 : 200	1 h RT	Tris-EDTA pH 9, 15 min	1 : 2500
TXNDC6	NBP2-15152; Novus Biologicals, Littleton, CO, USA	1 : 250	1 h RT	Tris-EDTA pH 9, 10 min	1 : 1000
8-OHdG	MOG-100P, anti-8-hydroxy-2-deoxyguanosine (N45.1); JaICA, Fukuroi, Japan	1 : 70	1 h RT	Citrate buffer pH 6, 15 min	—
Beta-actin	NB600-501; Novus Biologicals, Cambridge, UK	—	—	—	1 : 20 000
PCNA	#2586, Cell signaling Technology, Danvers, Ma, USA				1 : 2000
Anti-rabbit HRP-linked	Cell Signaling Technology, Danvers, MA, USA	—	—	—	1 : 3000
Anti-mouse HRP-linked	Cell Signaling Technology, Danvers, MA, USA	—	—	—	1 : 5000