circ_0023461 Silencing Protects Cardiomyocytes from Hypoxia-Induced Dysfunction through Targeting miR-370-3p/PDE4D Signaling

Figure 2

circ_0023461 silencing protects cardiomyocytes against hypoxia-induced dysfunction. (a) The transfection efficiency of si-circ_0023461 was analyzed in AC16 cells by RT-qPCR. (b–l) AC16 cells were divided into four groups: normoxia, hypoxia, hypoxia+si-NC, and hypoxia+si-circ_0023461. (b) Cell viability was analyzed by CCK8 assay. (c) Cell proliferation ability was evaluated by Edu assay. (d) Cell migration ability was assessed by Transwell migration assay. (e) Flow cytometry was performed to analyze cell apoptosis rate. (f, g) Cell oxidative stress was analyzed through measuring the content of MDA and the activity of SOD using the commercial kits. (h) The release of proinflammatory cytokines (IL-6 and TNF-α) was analyzed by ELISA assay. (i–k) Cell glycolytic metabolism was analyzed through detecting the uptake of glucose and the production of lactate and ATP using the colorimetric assay kits. (l) The protein levels of glycolysis-associated proteins (HK2 and LDHA) were measured by Western blot assay. .

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