circ_0023461 Silencing Protects Cardiomyocytes from Hypoxia-Induced Dysfunction through Targeting miR-370-3p/PDE4D Signaling

Figure 4

circ_0023461 silencing-mediated protective effects in hypoxia-induced cardiomyocytes are partly overturned by the knockdown of miR-370-3p. (a) RT-qPCR was conducted to analyze the transfection efficiency of anti-miR-370-3p in AC16 cells. (b–l) AC16 cells were divided into the following four groups: hypoxia+si-NC, hypoxia+si-circ_0023461, hypoxia+si-circ_0023461+anti-miR-NC, and hypoxia+si-circ_0023461+anti-miR-370-3p. (b) CCK8 assay was conducted to analyze cell viability. (c) Cell proliferation was analyzed by Edu assay. (d) Cell migration ability was analyzed by Transwell migration assay. (e) Cell apoptosis rate was evaluated by flow cytometry. (f, g) The content of MDA and the activity of SOD were measured using the commercial kits. (h) ELISA assay was conducted to analyze the production of proinflammatory cytokines (IL-6 and TNF-α) in the supernatant. (i–k) The consumption of glucose and the levels of lactate and ATP were measured using the colorimetric assay kits. (l) The protein levels of HK2 and LDHA were measured by Western blot assay. .

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