Research Article

Transcriptional Profiling of Exosomes Derived from Staphylococcus aureus-Infected Bovine Mammary Epithelial Cell Line MAC-T by RNA-Seq Analysis

Table 3

Summary of filter data.

Sample nameRaw readsRaw basesClean readsGC%Q20 (%)Q30 (%)Clean basesClean bases%

cont167572136101358204006565005648.5799.1697.32968901723495.59
cont26647470799712060506500637047.5499.2297.52951459664395.42
infect16903329398549939506578988749.6798.9596.74938392523995.22
infect270292999105439498506882609249.9198.9496.691010220809695.81

Raw reads: statistics of raw reads, each adjacent four lines contains the information of one read, and the total read number of each file is calculated; raw base: the number of all bases in raw data; clean reads: filter the original data, remove the linker sequence, remove the contaminated part, and remove the sequence containing more low-quality bases to obtain clean reads; GC%: the percentage of G and C in all bases; Q20, Q30: the percentage of total number of bases where the Phred score is greater than 20 and 30 which indicates base call accuracy; clean bases: the number and length of sequences in clean reads calculate the number of bases; clean bases%: percentage of clean bases/raw base.