Transcriptional Profiling of Exosomes Derived from Staphylococcus aureus-Infected Bovine Mammary Epithelial Cell Line MAC-T by RNA-Seq Analysis

Table 4

Summary of reads mapped to reference genome.

Sample name	cont1	cont2	infect1	infect2
The effective reads	112941906 (100%)	123138246 (100%)	86025798 (100%)	107088594 (100%)
Total mapped	5194592 (4.60%)	3683147 (2.99%)	4047510 (4.70%)	3269932 (3.05%)
Multiple mapped	550374 (0.49%)	253166 (0.21%)	492395 (0.57%)	411581 (0.38%)
Uniquely mapped	4644218 (4.11%)	3429981 (2.79%)	3555115 (4.13%)	2858351 (2.67%)
Read1 mapped	2600145 (2.30%)	1843109 (1.50%)	2022180 (2.35%)	1633528 (1.53%)
Read2 mapped	2594447 (2.30%)	1840038 (1.49%)	2,025,330 (2.35%)	1636404 (1.53%)
Reads map to “+”	2,599,952 (2.30%)	1842749 (1.50%)	2025784 (2.35%)	1636402 (1.53%)
Reads map to “-”	2594640 (2.30%)	1840398 (1.49%)	2,021,726 (2.35%)	1633530 (1.53%)
Reads mapped in proper pairs	4815452 (4.26%)	3470648 (2.82%)	3800530 (4.42%)	3059418 (2.86%)

The effective reads: the remaining number of clean reads after removing rRNA reads will be used for subsequent genome alignment; total mapped: the number of sequencing sequences that can be aligned on the genome; multiple mapped: the number of sequencing sequences with multiple alignment positions on the sequence of reference; uniquely mapped: the number of sequencing sequences with unique alignment positions on the reference sequence; read-1, read-2 mapped: pair-end sequence, whose two parts that can be located on the number of genome, respectively; the statistical ratio of the two parts should be roughly the same; reads map to “+”: the number of reads aligned to the positive strand of the genome; reads map to “-”: the number of reads aligned to the genome on the negative strand; reads mapped in proper pairs: the relative distance of the pair end sequence mapping to the genome conforms to the length distribution of the sequenced fragments.