Dihydrolycorine Attenuates Cardiac Fibrosis and Dysfunction by Downregulating Runx1 following Myocardial Infarction

Figure 4

Identification of dihydrolycorine binding proteins. (a) Protein expression with representative gel blots of Runx1 according to western blotting. Lysates prepared from (a) rats’ ventricle tissues and (b) H9C2 cells were added to the streptavidin-agarose beads with Bio-Dihy that were previously added to the streptavidin-agarose beads with prior incubation. (c) The upstream signaling proteins of Runx1 according to GeneCards and the STRING Interaction Network. (d) Pull-down assay was used to assess dihydrolycorine binding to Runx1, CBFB, and CEBPA. Lysates were obtained from primary cardiomyocytes. (e–g) Representative images were obtained by molecular docking between dihydrolycorine and Runx1.

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