Acteoside Counteracts Interleukin-1β-Induced Catabolic Processes through the Modulation of Mitogen-Activated Protein Kinases and the NFκB Cellular Signaling Pathway

Figure 2

Acteoside does not affect L929 mouse fibroblast cell and primary rat chondrocyte viability. (a) Acteoside did not affect the viability of L929 mouse fibroblast cells. (b) Acteoside did not decrease the viability of primary rat chondrocytes. Mouse fibroblast cell line L929 used as normal cells and primary rat chondrocytes were treated with 2.5, 5, 10, 25, 50, and 100 μM acteoside for 24 h. Thereafter, MTT assay was performed to assess the cytotoxicity of acteoside in L929 cells and primary rat chondrocytes. (c) Acteoside did not increase the cytotoxicity in both L929 and primary rat chondrocytes. To perform the Cell Live/Dead assay, L929 and primary rat chondrocyte were treated with 50 and 100 μM acteoside for 24 h. Thereafter, Cell Live/Dead assay was performed. Stained cells were imaged using a fluorescence microscope (Eclipse TE200; Nikon Instruments, Melville, NY).

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