Acteoside Counteracts Interleukin-1β-Induced Catabolic Processes through the Modulation of Mitogen-Activated Protein Kinases and the NFκB Cellular Signaling Pathway

Figure 3

Acteoside counteracts IL-1β-induced proteoglycan loss in primary rat chondrocytes. (a) Acteoside rescued the proteoglycan production in the primary rat chondrocytes treated with IL-1β. Primary rat chondrocytes embedded in alginate were treated with 50 and 100 μM acteoside in the presence or absence of 1 ng/mL IL-1β for 21 days. Thereafter, DMMB assay was performed to verify the alteration of proteoglycan contents. (b) Acteoside inhibits the IL-1β-induced proteoglycan loss in the articular cartilage dissected from rat knee joints. The tissues of articular cartilage were dissected from rat knee joint and were treated with 100 μM acteoside in the presence or absence of 10 ng/mL IL-1β for 7 days. Thereafter, histological assessment using safranin-O and fast green staining was performed to verify the alteration of proteoglycan loss. Stained tissues were imaged using a microscope (Eclipse TE200; Nikon Instruments, Melville, NY).

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