Acteoside Counteracts Interleukin-1β-Induced Catabolic Processes through the Modulation of Mitogen-Activated Protein Kinases and the NFκB Cellular Signaling Pathway

Figure 4

Acteoside has an anticatabolic effect that suppresses MMP expression and activation in primary rat chondrocytes treated with IL-1β. (a–c) Acteoside suppressed the expression of cartilage degrading enzymes such as MMP-13, MMP-1, and MMP-3 in the primary rat chondrocytes treated with IL-1β. Primary rat chondrocytes were treated with 50 and 100 μM acteoside in the presence or absence of 10 ng/mL IL-1β for 24 h. Thereafter, total proteins and total RNA were extracted to perform western blot (a), quantitative PCR (b), and quantitative real-time PCR (c). (d) Acteoside suppressed the activation of cartilage-degrading enzymes. Primary rat chondrocytes were treated with 50 and 100 μM acteoside in the presence or absence of 10 ng/mL IL-1β for 24 h. Thereafter, zymography was performed to verify the alteration of activated MMPs.

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