Acteoside Counteracts Interleukin-1β-Induced Catabolic Processes through the Modulation of Mitogen-Activated Protein Kinases and the NFκB Cellular Signaling Pathway

Figure 5

Acteoside suppresses the expression and production of IL-1β-induced catabolic inflammatory mediators in primary rat chondrocytes. (a, b) Acteoside suppressed the expression of inflammatory mediators in the primary rat chondrocytes treated with IL-1β. Primary rat chondrocytes were treated with 50 and 100 μM acteoside in the presence or absence of 10 ng/mL IL-1β for 24 h. Thereafter, total proteins and total RNA were extracted to perform quantitative PCR (a) and western blot (b). (c, d) Acteoside suppressed the production of NO (c) and PGE₂ (d) in the primary rat chondrocytes treated with IL-1β. Primary rat chondrocytes were treated with 50 and 100 μM acteoside in the presence or absence of 10 ng/mL IL-1β for 24 h. Thereafter, the NO assay (c) and PGE₂ assay (d) were performed on conditioned media.

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