Acteoside Counteracts Interleukin-1β-Induced Catabolic Processes through the Modulation of Mitogen-Activated Protein Kinases and the NFκB Cellular Signaling Pathway

Figure 8

Acteoside suppresses translocation of NFκB from the cytosol to the nucleus through suppression of IL-1β-induced NFκB phosphorylation in primary rat chondrocytes. (a) Acteoside suppressed the nucleus translocation of NFκB in the primary rat chondrocytes treated with IL-1β. Primary rat chondrocytes cultured on the chamber slide were treated with 50 and 100 μM acteoside in the presence or absence of 10 ng/mL IL-1β for 24 h. Thereafter, the nucleus translocation was imaged using a laser confocal scanning microscope system (Leica Microsystems, Wetzlar, Germany). (b) The translocation of NFκB from the cytosol to nucleus was suppressed by acteoside in primary rat chondrocytes treated with IL-1β. Primary rat chondrocytes were treated with 50 and 100 μM acteoside in the presence or absence of 10 ng/mL IL-1β for 24 h. Thereafter, cytosolic and nucleus proteins were extracted and western blot was performed.

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