Acteoside Counteracts Interleukin-1β-Induced Catabolic Processes through the Modulation of Mitogen-Activated Protein Kinases and the NFκB Cellular Signaling Pathway

Figure 9

Acteoside attenuates progressive degeneration of articular cartilage in the surgical DMM-induced knee joint OA animals. (a) Progressive degeneration of articular cartilage was attenuated by the oral administration of acteoside in the surgical DMM-induced knee joint OA animals. OA animals were generated by the DMM surgery on the knee joint and were orally administrated 5 and 10 mg/kg acteoside resolved in 5% ethanol every other day for 8 weeks. At the end of day, knee joints were dissected, fixed, decalcified, embedded, and sliced to performed safranin-O and fast green staining. Tissues were imaged using a microscope (Eclipse TE200; Nikon Instruments, Melville, NY). (b) Acteoside counteracted the Mankin grading score in the surgical DMM-induced knee joint OA animals. After safranin-O and fast green staining, imaged tissues of articular cartilage were examined in accordance with the Mankin grade.