Exosomal circEhmt1 Released from Hypoxia-Pretreated Pericytes Regulates High Glucose-Induced Microvascular Dysfunction via the NFIA/NLRP3 Pathway

Figure 2

Exosomal circEhmt1 was upregulated in hypoxia-pretreated pericytes. (a) Exosome morphology was observed by TEM. (b) The exosome surface markers TSG101, CD63, and CD81 were analyzed by western blot analysis. (c) All expressed exosomal circRNAs were classified. (d) Heat map representation of the top 10 differently expressed exosomal circRNAs. The circular structure of circEhmt1 was verified by (e) RNase R treatment and (f) divergent primer PCR; cDNA: complementary DNA; gDNA: genomic DNA. C1, C2, C3: samples from control. H1, H2, H3: samples from hypoxia cells.