RETRACTED: Resveratrol Derivative, Trans-3, 5, 4-Trimethoxystilbene Sensitizes Osteosarcoma Cells to Apoptosis via ROS-Induced Caspases Activation

Figure 1

TRAIL suppresses viability and promotes apoptotic cell death in Saos-2 cells. MTT assay (a) and Trypan blue staining (20x objective lens) (b) were used to examine the survival of 143B and Saos-2 cells after treatment with 0–200 ng/mL TRAIL for 12 h. Equal volume of vehicle (0.01% BSA + PBS) was used to treat the control groups, ; . (c) Western blotting was used to detect PRAP-1 cleavage in 143B and Saos-2 cells treated with 0–200 ng/mL TRAIL for 6 hour. (c) DAPI and TUNEL labeling were used to examine apoptosis in 143B and Saos-2 cells treated with 100 ng/mL TRAIL for 6 hours. White arrows indicate chromatin condensation in 143B and Saos-2 cells; spots of green fluorescence indicate morphological changes in 143B and Saos-2 cells (magnification ×200); additional higher-magnification images are also provided (magnification ×400).

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