RETRACTED: Resveratrol Derivative, Trans-3, 5, 4-Trimethoxystilbene Sensitizes Osteosarcoma Cells to Apoptosis via ROS-Induced Caspases Activation

Figure 2

TMS reverses TRAIL-resistance in 143B cells. (a) After treatment with different doses of TMS for 6 h, the survival rate of 143B cells was examined using the Trypan blue dye exclusion method (20x objective lens). (b) Survival of 143B cells, treated with 0–10 μm TMS and/or 50 ng/mL TRAIL, was assessed using the Trypan blue dye exclusion method. (c) Human osteosarcoma 143B cells and (d) normal human hFOB1.19 osteoblasts were pretreated with 50 ng/mL TRAIL and 2.5 μm TMS for 10 hours. Western blotting was used to detect the levels of cleaved PARP1. (e) DAPI and TUNEL labeling were used to evaluate the apoptosis of 143B osteosarcoma cells and normal hFOB1.19 osteoblasts after treatment with TMS and/or TRAIL for 6 h (magnification ×400).

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