RETRACTED: Resveratrol Derivative, Trans-3, 5, 4-Trimethoxystilbene Sensitizes Osteosarcoma Cells to Apoptosis via ROS-Induced Caspases Activation

Figure 3

Cotreatment with TRAIL and TMS can activate caspases in 143B cells. (a) Western blotting was used to detect the expression of PARP-1 and caspase cleavage in 143B cells treated with TMS and/or TRAIL for different lengths of time. The control group was treated with an equal volume of 1% DMSO + PBS+0.01% BSA. (b) Caspase cleavage was examined by Western blotting in 143B cells after treatment with 50 ng/mL TRAIL and TMS (0.5–2.5 μM) for 10 hours. (c) Caspase inhibitory agents, including zlEHDfmk, zlETDfmk, and zDEVDfmk, were used to block the caspase pathway in 143B cells. After the cells were pretreated with TMS and/or TRAIL for 10 hours, the Trypan blue dye exclusion method was used to evaluate cell survival (20x objective lens), and Western blotting was utilized to detect the expression of cleaved PARP1. .

(a)

(b)

(c)