RETRACTED: Resveratrol Derivative, Trans-3, 5, 4-Trimethoxystilbene Sensitizes Osteosarcoma Cells to Apoptosis via ROS-Induced Caspases Activation

Figure 4

TMS increases ROS levels in 143B cells. (a) Intracellular ROS levels in 143B cells were detected using fluorescence microscopy. The cell was pretreated with positive control (H₂O₂) or TMS for 50 minutes and labeled with CMH2DFCDA for 15 minutes. Considerably higher fluorescence intensity was detected in cells treated with H₂O₂ or TMS compared with that of the 0.01% BSA+ PBS-treated control group. (b) 143B cell was pretreated with different doses of TMS for various periods of time. ROS generation was measured using relative fluorescence intensity in 143B cells. . (c) After treatment with TMS (2.5, 5 μm) for various periods of time, 143B cell was labeled with CMH2DFCDA. ROS levels were analyzed at different time points according to the value of relative fluorescence intensity. (d) Pretreatment with NAC reduced ROS generation induced by TMS (2.5 μm) and (e) attenuated TMS- and TRAIL-induced apoptosis in 143B cells. Trypan blue dye exclusion method was used to evaluate cell survival (20x objective lens). ; . Cells in the control group were treated with an equal volume of PBS+0.1% DMSO . (f) Immunoblotting were used for examining the expression of cleaved PARP-1, p53, and PUMA in 143B cells after pretreatment with NAC and treatment with TRAIL and TMS.

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