RETRACTED: Resveratrol Derivative, Trans-3, 5, 4-Trimethoxystilbene Sensitizes Osteosarcoma Cells to Apoptosis via ROS-Induced Caspases Activation

Figure 5

TMS induces DNA damage and facilitates phosphorylation of Ser-139 (H2AX) in 143B cells. (a) Comet assay was performed to examine DNA damage induced by treatment with TMS or TRAIL for 15 minutes. Tail moment were applied for quantifying double-strands break via calculating the tail length × percentage of tail DNA. . (b) 143B cell was treated with 0–10 μM TMS for 24 hours, and Western blotting was used to examine the expression of H2AX and phospho-H2AX-Ser139 in 143B cells; 0.01% DMSO was used as vehicle control. (c) 143B cell was pretreated with TMS (2.5 μM) for 0-24 hours, and immunoblotting was used to examine the expression of H2AX and phospho-H2AX-Ser139 expression in 143B cells; 0.01% DMSO was used as vehicle control.

(a)

(b)

(c)