RETRACTED: Resveratrol Derivative, Trans-3, 5, 4-Trimethoxystilbene Sensitizes Osteosarcoma Cells to Apoptosis via ROS-Induced Caspases Activation

Figure 6

TMS activates PUMA and p53 expression in osteosarcoma cells. (a) TMS (2.5 μM) was used to treat 143B cells for 0–24 h, and protein expression of phosphorylated p53 (on Ser392, Ser46, or Ser15) was examined by Western blotting; 0.01% DMSO was used as vehicle control. (b) Different doses of TMS were used to treat 143B cells for 10 h, and Western blot assay was performed to detect the expressions of P53 and PUMA. (c) Different doses of TMS and TRAIL were used to treat 143B cells for 10 h, and Western blot assay was performed to detect the expressions of P53 and PUMA. (d) Gene expression of p53 and PUMA was silenced by siRNA in 143B cells, and then, the cells were treated with TRAIL and TMS for 10 h. Western blotting was used to examine the expression of cleaved PARP1, P53, and PUMA. (e) Apoptotic cells were examined by DAPI staining after treating p53- or PUMA-silenced 143B cells with TMS and TRAIL. White arrows indicate condensed chromatin in 143B cells (magnification ×400).

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