RETRACTED: Resveratrol Derivative, Trans-3, 5, 4-Trimethoxystilbene Sensitizes Osteosarcoma Cells to Apoptosis via ROS-Induced Caspases Activation

Figure 8

The role of BAX in TMS- and TRAIL-mediated apoptosis. (a, b) 143B Bax+/− and 143B Bax−/− cells were exposed to TMS and TRAIL for 10 h. Equal volume of 0.01% BSA + PBS+0.1% DMSO was used as vehicle control. Cell survival rate was analyzed as indicated in the experimental method section. Immunoblotting was used to examine the expression of cleaved PARP1, P53, Bax, and PUMA. . (c) DAPI was used as specific counterstain for apoptosis to evaluate the effects of cotreatment with TMS and TRAIL in 143B Bax+/− and 143B Bax−/− cells. White arrows indicate chromatin condensation in 143B cells evaluated by fluorescence microscopy (magnification ×400).

(a)

(b)

(c)