Farrerol Enhances Nrf2-Mediated Defense Mechanisms against Hydrogen Peroxide-Induced Oxidative Damage in Human Retinal Pigment Epithelial Cells by Activating Akt and MAPK

Figure 3

Inhibitory impact of farrerol on H₂O₂-induced apoptosis in vitro. (a, b) Quantitation of apoptotic cells with flow cytometry. (c) Levels of the Bax/Bcl-2 (d), caspase-3 (e), cleaved PARP/pro-PARP (f), cleaved caspase-8 (g), and cleaved caspase-9 (h) proteins were assessed utilizing western blotting. The mRNA expression of Bax (i) and Bcl-2 (j) in ARPE cells treated with H₂O₂ (300 μM) and different concentrations of farrerol (5, 10, and 20 mg/L). All of the data represent the means of three independent experiments. and compared with the H₂O₂ group. β-Actin was used as an internal control.

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