Farrerol Enhances Nrf2-Mediated Defense Mechanisms against Hydrogen Peroxide-Induced Oxidative Damage in Human Retinal Pigment Epithelial Cells by Activating Akt and MAPK

Figure 5

Nrf2 knockdown weakens the protective effect of farrerol in vitro. Cells were transfected with an Nrf2 siRNA or Nrf2-negative control siRNA for 1 h and exposed to farrerol for 24 h, and the protein levels were subsequently detected with western blotting. (a, b) The levels of Nrf2 and HO-1 were determined. (c) Analysis of the cytoprotective effect of farrerol using the CCK-8 assay. Immunoblotting (d) and quantification of Bax/Bcl-2 (e), cleaved PARP (f), cleaved caspase-3 (g), cleaved caspase-8 (h), and cleaved caspase-9 (i) protein expression following transfection with siNrf2. All results are presented as (). and compared with the H₂O₂ group. NS = not specific. β-Actin served as an internal control.

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