Effect of Vitamin D Status on Vascular Function of the Aorta in a Rat Model of PCOS

Figure 2

(a) Resorcin-fuchsin staining in aortic tissue. Conventional resorcin-fuchsin staining was applied to aortic rings then noncalibrated optical density was measured. A significantly lower staining density was detected in T-D- group compared to the control. in each group, Kruskal-Wallis test, Dunn’s multiple comparisons test. bb: T-D- vs. T-D+ . Data are presented as individual data points; lines represent median [IQR]. (b) Immunohistochemical measurement of 3-nitrotyrosine (NT) staining intensity in the aortae of female rats. NT immunostaining is visualized by brown-colored DAB, while hematoxylin is used as counterstaining. We detected significantly elevated staining density in T-D- rats compared to controls. in each group, Kruskal- Wallis test, Dunn’s multiple comparisons test. b: T-D- vs. T-D+ . Data are presented as individual data points; lines represent median [IQR].

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