Bilirubin Oxidation End Products (BOXes) Induce Neuronal Oxidative Stress Involving the Nrf2 Pathway

Figure 5

BOX A or BOX B trigger antioxidant responsive gene expression. (a) Top pathways analysis of BOX A or BOX B-treated PCNs showing the differentially expressed genes (DEGs) by microarray screening with criteria and value < 0.05 by using online tool GeneAnalytics (http://geneanalytics.genecards.org). (b) Heatmap analysis of significantly altered DEGs (see the supplemental Table 1). (c and d) RT-qPCR validation of the DEGs screened by microarray. Fold changes were interpreted as ( triplicates, one representative of 3 independent experiments), ANOVA with post hoc test. , , , compared to Mock (0.1% DMSO). (b–d) Genes are abbreviated: Nrf2: NF-E2 related factor 2; Hmox1: heme oxygenase 1; Nqo1: NADPH quinone oxidoreductase 1; Blvrb: biliverdin reductase B (flavin reductase (NADPH)); Gclm: glutamate-cysteine ligase, modifier subunit; Gsta3: glutathione S-transferase, alpha 3; Srxn1: sulfiredoxin 1 homolog (S. cerevisiae).