Downregulation of miR-128 Ameliorates Ang II-Induced Cardiac Remodeling via SIRT1/PIK3R1 Multiple Targets

Figure 1

Effects of silencing miR128 on the cardiac function and histomorphology in the mice with heart failure induced by chronic Ang II delivery. (a) Representative images of M-mode echocardiography in the mice, . (b) Representative images of the hearts and HE staining in the mice (left panel) and summary of cardiac mass index (upper right panel, ) and cardiomyocyte cross-sectional area (lower right panel, ). (i) scale bar, 100 μm, magnification, ×40, (ii) 500 μm, ×40, and (iii) 100 μm, ×100. (c) Masson staining of the left ventricular of the mice (left panel) and quantification of CVF (right panel, ). (i) scale bar, 100 μm, ×40 and (ii) scale bar, 100 μm, ×100. (d) Representative images of the expression of AAV2/9-miR-128-GFP adeno-associated virus sponge in the mouse cardiomyocytes (left panel, ) and the levels of miR-128 expression in the left ventricle of mice (right panel, ), 100 μm, ×100. HW: heart weight; BW: body weight. Data are expressed as . Statistical significance was assessed by using one-way ANOVA followed by LSD. , vs. control group; ^#, ^## vs. Ang II group.

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