Downregulation of miR-128 Ameliorates Ang II-Induced Cardiac Remodeling via SIRT1/PIK3R1 Multiple Targets

Figure 2

Effect of downregulation of miR-128 on oxidative stress in vivo and in vitro. NRCMs: neonatal rat cardiomyocytes. (a) The MDA contents (left panel) and SOD activities (right panel) in the left ventricle (LV) of mice. (b) Levels of the miR-128 expression at the different time points in H9c2 treated with Ang II or H₂O₂. (c) The expression level of miR-128 in H9c2 cells and NRCMs (left panel) as well as contents of MDA and SOD (right panel) after 12 h treatment with Ang II. (d) Representative images of ROS-sensitive DCF fluorescence (left panel) and ROS levels in H9c2 cells (right panel). Scale bar, 50 μm, magnification, ×200. Data are expressed as . Statistical significance was assessed by using one-way ANOVA followed by LSD. In the experiments of mice, , , vs. control group; ^#, ^## vs. Ang II group. In the experiments of cells, independent batches, repeat 2 ~ 3 wells/each batch cells, , vs. 0 h; , vs. control group; ^#, ^## miR-128 antag + Ang II vs. NC + Ang II group.

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