Downregulation of miR-128 Ameliorates Ang II-Induced Cardiac Remodeling via SIRT1/PIK3R1 Multiple Targets

Figure 3

Effect of miR-128 knockdown on cardiomyocytes apoptosis induced by Ang II. (a) Expression levels of Bcl2 and Bax proteins in the LV of mice. (1) Control, (2) Ang II, and (3) miR-128 inhibitor+Ang II. , , vs. control group; ^#, ^## vs. Ang II group. (b) Hoechst33258 fluorescent staining of H9c2 cells (apoptotic cells indicated by yellow arrows), scale bar, 50 μm, magnification, ×200. (c) Quantification of apoptosis rate (left panel) and survival rate of H9c2 cells (right panel, MTT method) of H9c2 cells. (d, e) Expression levels of Bcl2 and Bax mRNA and proteins in H9c2 cells. In cell experiments: independent batches, repeated 5 wells/each batch for the survival rate and repeat 2 ~ 3 wells/each batch cells for the remaining experiments. Data are expressed as . Statistical significance was assessed by using one-way ANOVA followed by LSD. , vs. control group; ^#, ^## miR-128 antag+Ang II vs. NC + Ang II group.

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