Downregulation of miR-128 Ameliorates Ang II-Induced Cardiac Remodeling via SIRT1/PIK3R1 Multiple Targets

Figure 4

Silencing miR-128 attenuates AngII-induced apoptosis through SIRT1/p53 pathway. (a) Expression levels of SIRT1 and P53 protein in the LV of mice. (1) Control, (2) Ang II, and (3) miR-128 inhibitor+Ang II. , , vs. control group; ^#, ^## vs. Ang II group. (b) Expression levels of SIRT1and P53 mRNA in H9c2 cells. (c) Expression levels of SIRT1 and p53 protein in H9c2 cells. (d) Representative images of the flow cytometry assay for apoptotic cells and quantification of apoptosis rate in H9c2 cells. independent batches and repeated 2 ~ 3 wells/each batch cells. Data are expressed as . Statistical significance was assessed by using one-way ANOVA followed by LSD. , vs. control; ^#, ^## vs. NC + Ang II; ^∆∆ vs. Ang II + miR-128 antag.

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