Downregulation of miR-128 Ameliorates Ang II-Induced Cardiac Remodeling via SIRT1/PIK3R1 Multiple Targets

Figure 5

Effect of miR-128 downregulation on Ang II-induced autophagy. (a) Expression levels of beclin 1, LC3 II, and p62 proteins in the LV of mice. , , vs. control group; ^#, ^## vs. Ang II group. (b) Representative images of the ultrastructure of H9c2 cells showing the changes of autophagy under a transmission electron microscope. Scale bar in (i), 2 μm and magnification, ×10000; in (ii), 1 μm, ×20000. Yellow arrow refers to the autophagosome, which contains dense electronic content such as autolysosomes or mitochondria. (c) Expression of beclin 1 and LC3 mRNA in H9c2 cells. (d, e) Expression of beclin 1, LC3II, and P62 proteins in H9c2 cells. In cell experiments, (1) control, (2) Ang II, (3) NC, (4) NC + Ang II, (5) miR-128 antag, (6) miR-128 antag + Ang II, (7) Ang II + CQ (chloroquine), and (8) miR-128 antag + Ang II + CQ. independent batches, repeated 2 ~ 3 wells/each batch. Data are expressed as . Statistical significance was assessed by using one-way ANOVA followed by LSD. , vs. control group; ^#, ^## vs. NC + Ang II group; ^∆, vs. Ang II group; ^▴ vs. Ang II + CQ group.

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