Downregulation of miR-128 Ameliorates Ang II-Induced Cardiac Remodeling via SIRT1/PIK3R1 Multiple Targets

Figure 6

Autophagy inhibition of miR-128 downregulation reduces Ang II-induced apoptosis and ROS production. NRCMs: neonatal rat cardiomyocytes. (a) Representative images of DHE staining for ROS and DAPI nuclear staining and summary of ROS levels in H9c2 cells. Scale bar: 50 μm, ×200. (b) Representative images of the flow cytometry assay for apoptotic cells and quantification of apoptosis rate in H9c2 cells. (c) Representative images of ROS-sensitive DCF fluorescence and summary of ROS levels in NRCMs. Scale bar: 100 μm, ×100. (d) Representative images of the flow cytometry assay for apoptosis and quantification of the apoptosis rate in NRCMs. independent batches, repeated 2 ~ 3 wells/each batch. Data are expressed as . Statistical significance was assessed by using one-way ANOVA followed by LSD. , vs. control group; ^#, ^## vs. Ang II group; ^∆∆ vs. Ang II + miR-128 antag group.

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