Study on Protection of Human Umbilical Vein Endothelial Cells from Amiodarone-Induced Damage by Intermedin through Activation of Wnt/β-Catenin Signaling Pathway

Figure 1

IMD protects HUVECs from amiodarone-induced proliferation, migration, and apoptosis. (a) HUVECs were treated with different concentrations of IMD, and cell viability was assessed via CCK-8 assay. (b) HUVECs were pretreated with different concentrations of IMD, followed by the stimulation of amiodarone; then, the cell viability was assessed via CCK-8 assay. (c, d) After pretreatment with IMD (20 ng/mL) for 2 h, HUVECs were incubated with amiodarone (30 μmol/L) for 24 h. The wound healing assay was performed to test cell migration. (e) The ΔΨm was detected by the JC-1 assay. (f) Flow cytometry analysis was performed to determine the apoptosis rate of each group. (g, h) The protein expressions Bcl-2, Bax, and caspase-3 were detected by western blotting analysis. (i) The mRNA levels of Bcl-2, Bax, and caspase-3 were detected by RT-PCR. Data was presented as the (SD) (; and vs. control).

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