Study on Protection of Human Umbilical Vein Endothelial Cells from Amiodarone-Induced Damage by Intermedin through Activation of Wnt/β-Catenin Signaling Pathway

Figure 2

IMD protects HUVECs from amiodarone-induced inflammation and oxidative stress. (a, b) The expression of proinflammatory cytokines, including IL-1β, IL-6, and TNF-α, and anti-inflammatory IL-10 was detected by western blotting. (c) The mRNA levels of IL-1β, IL-6, TNF-α, and IL-10 were detected by RT-PCR. (d) The production of ROS, the release of LDH, and the vitality of SOD, GSH-Px, and CAT were detected using the standard assay. (e) The expression of antioxidant genes (SOD1, SOD2, GSH-Px1, and CAT) was detected by RT-PCR. Data was presented as the mean ± standard deviation (SD) (; , vs. control).

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