Study on Protection of Human Umbilical Vein Endothelial Cells from Amiodarone-Induced Damage by Intermedin through Activation of Wnt/β-Catenin Signaling Pathway

Figure 3

IMD activates the Wnt signaling pathway. (a, b) After treatment with different concentrations of amiodarone, the levels of β-catenin and p-β-catenin in HUVECs were determined by western blotting. (c, d) HUVECs were pretreated with IMD (20 ng/mL) for 2 h and then exposed to amiodarone (30 μmol/L) for 24 h. The protein expression of β-catenin and p-β-catenin was detected by western blotting. (e–g) HUVECs were pretreated with IWR (10 μmol/L) for 2 hours and then stimulated with amiodarone for 24 hours, and the expression levels of β-catenin and p-β-catenin were detected by western blotting. The mRNA levels were detected by RT-PCR. (h, i) After pretreatment with IMD (20 ng/mL) and IWR (10 μmol/L) for 2 h, HUVECs were incubated with amiodarone for 24 h. The expression of β-catenin and p-β-catenin was detected by western blotting. Data was presented as the mean ± SD (; and vs. control).

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