Study on Protection of Human Umbilical Vein Endothelial Cells from Amiodarone-Induced Damage by Intermedin through Activation of Wnt/β-Catenin Signaling Pathway

Figure 4

IWR abolishes the protective effects of IMD in HUVECs. HUVECs were pretreated with IMD (20 ng/mL) and IWR (10 μmol/L) for 2 h and then incubated with amiodarone for 24 h. (a) Cell viability was assessed via CCK-8 assay. (b, c) The wound healing assay was performed to test cell migration. (d) The ΔΨm was detected by the JC-1 assay. (e–j) The protein expressions of Bcl-2, Bax, and caspase-3 were detected by western blotting analysis. (k) The mRNA levels of Bcl-2, Bax, and caspase-3 were detected by RT-PCR. Data was presented as the (; and vs. control).

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)

(j)

(k)