Study on Protection of Human Umbilical Vein Endothelial Cells from Amiodarone-Induced Damage by Intermedin through Activation of Wnt/β-Catenin Signaling Pathway

Figure 6

Wnt/β-catenin siRNA transfection in HUVECs inhibits the protective effects of IMD. The siRNA was used to specifically knock down the β-catenin in HUVECs, and the blank carrier was used as a negative control. After transfection, cells were divided into five groups: si NC group, si NC+AM group, si NC+IMD group, siRNA+AM group, and siRNA+IMD group. (a–c) The efficiency of β-catenin knockdown by its siRNA in HUVECs was assessed by western blotting and RT-PCR. (d) Cell viability was assessed via CCK-8 assay after transfection. (e, f) The protein expressions of Bcl-2, Bax, and caspase-3 were detected by western blotting analysis. (g) Flow cytometry analysis was performed to determine the apoptosis rate of each group. (h) The mRNA levels of Bcl-2, Bax, and caspase-3 were detected by RT-PCR. Data was presented as the (SD) (; and vs. control).

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