Oxidative Medicine and Cellular Longevity

Research Article

Effect of Maternal Marginal Zinc Deficiency on Development, Redox Status, and Gene Expression Related to Oxidation and Apoptosis in an Avian Embryo Model

Table 2

Correlation between the measured parameters of Zn supply in the breeders and embryos.


Item	Breeder stage¹				Embryonic stage²
Item	Embryonic mortality	Plasma Zn concentration	Erythrocytic ALP activity	Erythrocytic 5-NT activity	MDA content	MT content	GSH-Px activity	GPx mRNA	MT1 mRNA	BCL2 mRNA

Embryonic mortality	1.00
Plasma Zn concentration	-0.30	1.00
Erythrocytic ALP activity	-0.42	0.71	1.00
Erythrocytic 5-NT activity	-0.50	0.75	0.61	1.00
MDA content	0.81	-0.55	-0.36	-0.54	1.00
MT content	-0.50	0.26	0.63	0.39	-0.36	1.00
GSH-Px activity	-0.45	0.80	0.60	0.68	-0.58	0.22	1.00
GPx mRNA	-0.39	0.63	0.54	0.69	-0.43	0.44	0.70	1.00
MT1 mRNA	-0.40	0.75	0.70	0.65	-0.49	0.47	0.74	0.88	1.00
BCL2 mRNA	-0.37	0.60	0.49	0.68	-0.40	0.54	0.67	0.92	0.87	1.00

¹Embryonic mortality, plasma Zn concentration, erythrocytic ALP, and 5-NT activity were measured in breeders at week 6 during experimental period. ²The MDA and MT contents, GSH-Px activity, and target gene mRNA expressions were determined in embryonic livers on E29. ALP: alkaline phosphatase; 5-NT: 5-nucleotidase; MDA: malondialdehyde; GSH-Px: glutathione peroxidase; GPx: glutathione peroxidase; MT1: metallothionein 1; BCL2: B-cell lymphoma-2.; .