miR-4454 Promotes Hepatic Carcinoma Progression by Targeting Vps4A and Rab27A

Figure 6

Vps4A and Rab27A, as miR-4454 target genes, were regulated by doxorubicin and TGF-β1 in HepG2 cells and exosomes. (a) After doxorubicin treatment, a Western blot analysis of Vps4A, Rab27A, Alix, and Hr was performed in HepG2 cells and exosomes. (b) After TGF-β1 treatment, Vps4A, Rab27A, Alix, and Hr levels were verified by Western blot assay in HepG2 cells and exosomes. (c, d) Binding sites between miR-4454 and Vps4A or Rab27A were predicted, and the regulations of miR-4454 in Vps4A and Rab27A were confirmed by the luciferase reporter gene assay.

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