miR-4454 Promotes Hepatic Carcinoma Progression by Targeting Vps4A and Rab27A

Figure 7

Inhibition of miR-4454 markedly suppressed proliferation and accelerated cycle arrest, apoptosis, and oxidative stress of HepG2 cells. HepG2 cells were transfected with the NC and miR-4454 inhibitor, respectively. (a–d) Cell proliferation was confirmed by CCK-8, EdU, and colony formation assays. (e) The apoptosis rate was determined by flow cytometry. (f) The cell cycle was examined by a flow cytometer. (g) ROS level was confirmed by the ROS assay kit. (h) Expressions of Vps4A, Rab27A, cleaved caspase-3, and Cyclin D1 were monitored by Western blotting. and vs. NC group; ^## for phase S versus NC group.

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