miR-4454 Promotes Hepatic Carcinoma Progression by Targeting Vps4A and Rab27A

Figure 8

Inhibition of miR-4454 dramatically inhibited migration, invasion, and vascularization of HepG2 cells. NC and miR-4454 inhibitors were applied to transfect HepG2 cells. (a, b) The Transwell assay was used to evaluate cell migration and invasion. (c) A wound healing assay was adopted to identify the ability of cells to migrate. (d) The angiogenic ability of cells was evaluated on Matrigel. and . (e) The expression of miR-4454 was examined by a qRT-PCR assay in HepG2 cells and exosomes after transfection with the miR-4454 inhibitor. and . (f) The levels of the downstream genes (Vps4A, Rab27A, TRPV6, and BAG5) were monitored by applying the qRT-PCR assay in HepG2 cells transfected with the NC and miR-4454 inhibitor, respectively. and .

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