miR-4454 Promotes Hepatic Carcinoma Progression by Targeting Vps4A and Rab27A

Figure 9

miR-4454 inhibitor-mediated exosomes prominently prevented proliferation and facilitated cycle arrest, apoptosis, and oxidative stress of HepG2 cells. Exosomes were extracted from NC or miR-4454 inhibitor-transfected HepG2 cells, and then, exosomes were applied to treat HepG2 cells. (a–d) CCK-8, EdU, and colony formation assays were adopted to examine the impacts of miR-4454 inhibitor-mediated exosomes on HepG2 cell proliferation (e, f). Cell apoptosis and cell cycle were surveyed by flow cytometry. (g) The ROS assay kit was used to test ROS level. (h) Western blot was utilized to determine the expressions of Vps4A, Rab27A, cleaved caspase-3, and Cyclin D1. , , and vs. NC/Exo group; ^## for phase S vs. NC/Exo group.

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