lncRNA NONHSAT069381 and NONHSAT140844 Increase in Aging Human Blood, Regulating Cardiomyocyte Apoptosis

Figure 2

Gene transfection of lncRNA (NONHSAT069381 and NONHSAT140844) and altered resultant caspase-3 and XIAP activity. Transfection efficiency, 48 hours after lncRNA (a) NONHSAT069381 and (b) NONHSAT140844 overexpression (OE-069381/OE-140844) or knockdown (KD-069381/KD-140844) by lentiviral transfection of AC16 cells. Transfection efficiency indicated by concomitant contrast and fluorescence microscopy. Lentiviral vectors carried GFP gene. AC16 cells infected by lncRNA NONHSAT069381/NONHSAT140844-carrying lentivirus are identifiable by fluorescence microscopy 48 hours after infection. (c, d) The relative expression of NONHSAT069381 was measured by RT-PCR. (e, f) Representative immunoblots of caspase-3 from NONHSAT069381 overexpression or knockdown in AC16 cells. (g, h) The relative expression of NONHSAT140844 was measured by RT-PCR. (i, j) Representative immunoblots of XIAP from NONHSAT140844 overexpression or knockdown in AC16 cells. . versus overexpression/knockdown control (OE-Ctrl/KD-Ctrl).

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