lncRNA NONHSAT069381 and NONHSAT140844 Increase in Aging Human Blood, Regulating Cardiomyocyte Apoptosis

Figure 3

Genetic manipulation of NONHSAT069381 and NONHSAT140844 alters AC16 cell apoptosis after H/R. (a, b) Representative photomicrographs of in situ detection of cardiomyocyte DNA fragments subjected to H/R. Cardiomyocytes were stained with TUNEL (red) and DAPI (blue). TUNEL-positive nuclei of the NONHSAT069381 group were summarized in graph and expressed as percentage of all cardiomyocytes subjected to H/R (c). Cardiomyocyte caspase-3, caspase-8, and caspase-9 activity of the NONHSAT069381 group was measured after H/R (d–f). TUNEL-positive nuclei and cardiomyocyte caspase-3, caspase-8, and caspase-9 activity of the NONHSAT140844 group (g–j). plates/group. versus overexpression/knockdown control.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)

(j)