lncRNA NONHSAT069381 and NONHSAT140844 Increase in Aging Human Blood, Regulating Cardiomyocyte Apoptosis

Figure 4

The miRNA-targeted mRNAs and lncRNA were validated by luciferase reporter assay. (a) Luciferase reporter assay performed by cotransfection of lncRNA NONHSAT069381 and luciferase reporter containing the wild or mutant 3-UTR of lncRNA NONHSAT069381 with hsa-miR-124-5p mimic or NC in 293T cells. (b) Luciferase reporter assay performed by cotransfection of luciferase reporter containing wild or mutant 3-untranslated repeat (UTR) of mCASP3 with hsa-miR-124-5p mimics or negative control in 239T cells. (c) Luciferase reporter assay performed by cotransfection of lncRNA NONHSAT140844 and luciferase reporter containing the wild or mutant 3-UTR of lncRNA NONHSAT140844 with hsa-miR-6507-5p mimic or negative control in 293T cells. (d) Luciferase reporter assay performed by cotransfection of luciferase reporter containing wild or mutant 3-untranslated repeat (UTR) of mXIAP with hsa-miR-6507-5p mimics or negative control in 239T cells. Luciferase activity was determined 48 hours after cotransfection. . versus mimic negative control.

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