lncRNA NONHSAT069381 and NONHSAT140844 Increase in Aging Human Blood, Regulating Cardiomyocyte Apoptosis

Figure 5

Gene transfection of miRNA (hsa-miR-124-5p and hsa-miR-6507-5p) and altered resultant caspase-3 and XIAP activity. (a) The relative expression of hsa-miR-124-5p was measured by RT-PCR. (b) The relative expression of mCASP3 from hsa-miR-124-5p overexpression (OE-hsa-miR-124-5p) or knockdown (KD-hsa-miR-124-5p) in AC16 cells confirmed by RT-PCR. (c, d) Representative immunoblots of caspase-3 from hsa-miR-124-5p overexpression or knockdown in AC16 cells. (e) The relative expression of NONHSAT069381 from hsa-miR-124-5p overexpression or knockdown in AC16 cells confirmed by RT-PCR. (f) The relative expression of hsa-miR-6507-5p was measured by RT-PCR. (g) The relative expression of mXIAP from hsa-miR-6507-5p overexpression (OE-hsa-miR-6507-5p) or knockdown (KD-hsa-miR-6507-5p) in AC16 cells confirmed by RT-PCR. (h, i) Representative immunoblots of XIAP from hsa-miR-6507-5p overexpression or knockdown in AC16 cells. (j) The relative expression of NONHSAT140844 from hsa-miR-6507-5p overexpression or knockdown in AC16 cells confirmed by RT-PCR. . versus overexpression/knockdown control.

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