Bortezomib Rescues Ovariectomy-Induced Bone Loss via SMURF-Mediated Ubiquitination Pathway

Figure 1

The effect of bortezomib on the osteogenic differentiation of MC3T3-E1 cells. (a) Representative images of mineralized extracellular matrix stained by Alkaline Phosphatase and Alizarin Red S staining. . (b) Quantification of ALP activity in bortezomib-treated MC3T3-E1 cells. (c) Quantification of stained mineral layers in bortezomib-treated MC3T3-E1 cells. The values were normalized to the level of the NC group. (d–h) The mRNA levels of osteoblast-specific marker genes, including Runx2, Col1a1, Alp, Sp7, and Bglap were quantified with real-time RT-PCR using GAPDH as the normalization. (i) The protein levels of osteoblast-specific marker enzymes including OPG, RUNX2, OSX, and OCN were determined using Western blot assays. Values represent of three replicas for ALP activity and ARS staining assays, three replicas for RT-PCR experiments and three replicas for Western blot assays, respectively. Statistically significant differences are indicated by where or where between the indicated groups.

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