Administration of Nrf-2-Modified Hair-Follicle MSCs Ameliorates DSS-Induced Ulcerative Colitis in Rats

Figure 1

Characteristics of HF-MSCs and establishment of Nrf-2-modified HF-MSCs. (a) After 72 h of culture, numerous HF-MSCs aggregated near the hair follicles (20x). (b) Third-generation HF-MSCs (20x). (c, d) Osteogenic and adipogenic differentiation of HF-MSCs. (e–l) Detection of CD44, CD90, CD45, and CD31 by flow cytometry. (m) Relationship between viral transduction efficiency and multiplicity of infection (MOI). Data are expressed as the . (n) Construction of the lentiviral Nrf-2 overexpression plasmid. (o) GFP-labeled Nrf-2 was transfected into DAPI-stained HF-MSCs, and obvious blue and green fluorescence was visible under the microscope (20x). (p) Western blot analysis of HF-MSCs transduced with Nrf-2-harboring virus construct.

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