Ghrelin Alleviates Endoplasmic Reticulum Stress in MC3T3E1 Cells by Inhibiting AMPK Phosphorylation

Figure 6

Expression of endoplasmic reticulum stress-related markers (BIP/GRP78, IRE1α, and CHOP) in different groups. (a)–(c) Histograms of the relative mRNA expression levels of BIP/GRP78, CHOP, and IRE1α after TM (1.5 μg/mL) treatment for 20 hours. (d)–(f) Histograms of the relative mRNA expression levels of BIP/GRP78, CHOP, and IRE1α after 4 h of preconditioning with ghrelin (10^-7 mol/L) and TM (1.5 μg/mL) treatment for 20 h. (g)–(i) Histograms of the relative mRNA expression levels of BIP/GRP78, CHOP, and IRE1α after GSK621 (10 μmol/L) pretreatment for 3 h, ghrelin (10^-7 mol/L) pretreatment for 4 h, and TM (1.5 μg/mL) treatment for 20 h. Data represents the obtained from three separate experiments. In (a)–(f), indicates comparison with the control group, ^# indicates comparison with the TM group, indicates , indicates ; ^## indicates , ^### indicates . In (g)–(i), indicates comparison with the TM group, ^# indicates comparison with the group, means , indicates , indicates , and ^### indicates .

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