Ghrelin Alleviates Endoplasmic Reticulum Stress in MC3T3E1 Cells by Inhibiting AMPK Phosphorylation

Figure 7

Relative expression levels of BIP, p-AMPK, and cleaved-caspase3 in different groups. BIP and cleaved-caspase3 band intensities were normalized to the β-actin intensity, and p-AMPK was normalized to the AMPK intensity. (a), a1-a3 are the grayscale image and histogram of the relative expression of BIP, p-AMPK, and cleaved-caspase3, respectively, after TM treatment for 20 hours. (b), b1-b3: The grayscale image and histogram of the relative expression levels of BIP, p-AMPK, and cleaved-caspase3 after 4 h of preconditioning with ghrelin (10^-7 mol/L) and TM (1.5 μg/mL) treatment for 20 h. (c), c1-c3: The grayscale image and histogram of the relative expression levels of BIP, p-AMPK, and cleaved-caspase3 after GSK621 (10 μmol/L) pretreatment for 3 h, ghrelin (10^-7 mol/L) pretreatment for 4 h, and TM (1.5 μg/mL) treatment for 20 h. Data represents the obtained from three separate experiments. In (a1)–(a3), 7b1-b3, indicates comparison with the control group, ^# indicates comparison with the TM group, indicates , indicates , ^## indicates ; In (c1)–(c3), indicates comparison with the TM group, ^# indicates comparison with the group, indicates , ^## indicates , and ^#### indicates .

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