Escherichia coli Aggravates Calcium Oxalate Stone Formation via PPK1/Flagellin-Mediated Renal Oxidative Injury and Inflammation

Figure 9

PPK1/flagellin may activate mouse renal inflammation via the NF-κB/P38 signaling pathway. (a) HK-2 treated with WT-CFT073 and 100 ng/mL flagellin increased the intracellular and nuclear phosphorylation of P38 and P65 (by Western blot, tubulin was used as an internal control, in comparison with the WT-CFT073 group). (b) MCP-1, p-P65, and p-P38 were upregulated in the Ga+WT-CFT073 group, but not other groups. Phosphorylation of P65 and P38 were located in both the cytoplasm and nuclear of renal tubular epithelial cells (original magnification: ×200, in comparison with the WT-CFT073 group).

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